Overview
- Peptide (C)KIQKKPGKSELRINK, corresponding to amino acid residues 87-101 of human Neuregulin-1 (Accession Q02297). Extracellular, N- terminus.
Expression of Neuregulin-1 in rat brainImmunohistochemical staining of rat brain sections using Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody (#ANR-111-AG), (1:80). Neuregulin-1 staining (green) in cerebellum appears in basket cell axonal formations (horizontal arrows) and axons in cerebellar white matter (vertical arrows). Nuclei are stained using DAPI as the counterstain (blue).
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ErbB receptors are a family of tyrosine kinase receptors encoded by the erbB gene. These receptors commence signaling pathways such as the mitogen activated protein kinase (MAPK) and inositol 3-kinase (PI3K) pathways and have an important role in the control of cell growth and survival1. Neuregulins are a family of EGF-like factors that are produced from alternative RNA splicing of the same gene. Through their interaction with ErbB receptors, Neuregulins regulate normal developmental processes, plasticity, and oncogenesis2.
Neuregulin-1 is one of the four known proteins of the Neuregulin family and can be found in many organs including the heart, breast, peripheral and central nervous system. Neuregulin-1 is encoded by a gene located on chromosome 8p13 which produces more than 15 different isoforms of the same protein. All isoforms contain a core EGF domain encoded by exon E130 but other elements of the protein vary. Most NRG1 isoforms are synthesized as transmembrane proproteins which undergo proteolytic cleavage processes3.
Due to its important function in the central and peripheral nervous system as well as in other organs NRG1 has been implicated in a variety of pathologies. The NRG1 gene is suspected as a schizophrenia susceptibility gene. Mice with a mutated NRG1 exhibit elevated activity in open field and abnormal sensorimotor gating which correspond with schizophrenic like behavior in humans. These affects are reversed using anti-psychotic drugs4. In addition, Activation of NRG1 was found to improve cardiac survival and function in a rat and canine model of dilated and ischemic hearts5.
Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and could potentially detect the protein in living cells. It has been designed to recognize Neuregulin-1 from rat, human, and mouse samples. This antibody will recognize NRG1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III.
Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody (#ANR-111-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.
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