Overview
- Peptide (C)SHITRRPNGKTWSTKR, corresponding to amino acid residues 656-671 of rat NLGN3 (Accession Q62889). Extracellular, N-terminus.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:1,2. Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037), (1:200).
3,4. Anti-Neuroligin 3 (extracellular) Antibody, preincubated with Neuroligin 3 (extracellular) Blocking Peptide (#BLP-NR037).
Expression of Neuroligin-3 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neuroligin-3 immunoreactivity (green) appears in in the granule layer (GL) and in some purkinje cells (arrows). B. Pre-incubation of the antibody with Neuroligin 3 (extracellular) Blocking Peptide (BLP-NR037), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Neuroligin-3 in rat deep cerebellar nuclei.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neuroligin-3 immunoreactivity (green) appears in deep cerebellar nuclei forming neuronal outlines (arrows). B. Pre-incubation of the antibody with Neuroligin 3 (extracellular) Blocking Peptide (BLP-NR037), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Hu, X. et al. (2015) Biomed Res. Int. 2015, 498957.
- Kalbassi, S. et al. (2017) eNeuro 4, e0145.
Neuroligins (Nlgns) are a family of postsynaptic transmembrane cell adhesion proteins that play a crucial role in excitation and inhibition balance through their regulation of GABAergic and glutamatergic signaling. 5 Neuroligin family members have been identified in humans: NL1, NL2, NL3, NL4X, and NL4Y. NL 1-3 have close homologs in mice. Neurogilins have been identified in rodents, chicken, Drosophila, and C. elegans.
Neuroligin structure contains extracellular acetylcholinesterase like domain that lacks esterase activity, a single transmembrane domain, and a short cytoplasmic tail containing a type I PDZ-domain-binding motif that functions in intracellular protein-protein interactions and signaling processes. Neuroligins and neurexins are known to interact with each other1,2.
Neuroligin 3 is encoded by the Nlgn3 gene and seems to be ubiquitously expressed in the brain. Despite this fact, Neuroligin 3 functions in synaptic transmission appears to be region specific, affecting the somatosensory cortex and the hippocampus differently2.
In humans, a missense mutation in Nlgn3 (Nlgn3R451C) causing reduced synaptic expression of the protein is associated with non-syndromic autism spectrum disorders. In addition, Nlgn3 deletion shows impaired social interaction and vocal communication in rodent models.
Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize NLGN3 from rat, mouse, and human samples.
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