Overview
- Peptide (C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1 (Accession P62168). Intracellular.
Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat eye (lanes 3 and 6) lysates (1:200-1:400):1-3. Anti-NCS1 Antibody (#ACS-001), (brain lysates 1:200, eye lysate 1:400).
4-6. Anti-NCS1 Antibody, preincubated with NCS1 Blocking Peptide (#BLP-CS001).
Expression of NCS1 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NCS1 Antibody (#ACS-001), (1:100), followed by goat anti-rabbit-Alexa-488. A. NCS1 immunoreactivity (green) appeared in the Purkinje layer (P, horizontal arrow) and in purkinje dendrites (vertical arrow) in the molecular layer (MOL). B. Pre-incubation of the antibody with NCS1 Blocking Peptide (BLP-CS001), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Pandalaneni, S. et al. (2015) J. Biol. Chem. 290, 18744.
- Bellucci, L. et al. (2013) PLoS One. 8, 74383.
- Heidarsson, P.O. et al. (2013) Structure. 21, 1812.
Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons.
The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.
NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.
NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Neuronal calcium sensor 1. Anti-NCS1 Antibody (#ACS-001) can be used in western blot analysis. It has been designed to recognize NCS1 from rat, mouse and human samples.
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