Name: Anti-Neuronal Pentraxin 1 (NPTX1) Antibody
Brand: Alomone Labs
SKU: ANR-191

Overview

Cat #: ANR-191

Alternative Name Neuronal Pentraxin-1, NP1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)ERAKIESALTSLHQR, corresponding to amino acid residues 197 - 211 of mouse NPTX1 (Accession Q62443). Secreted.

Accession (Uniprot) Number Q62443

Gene ID 18164

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical; Human - 13 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):
1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).

Immunohistochemistry

Expression of Neuronal Pentraxin 1 in rat striatum.
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NPTX1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191), suppressed staining. Cell nuclei are stained with DAPI (blue). CC = corpus callosum, ST = striatum.

References

Ma, Q.L. et al (2018) Neurobiol Dis, 114, 120.
Mir Ahamed Hossain (2008), Int J Dev Neurosci, 26, 93.
Wang, Z. et al (2020) Front Immunol, 11, 1757.

Scientific background

Neuronal Pentraxin 1 (NP1 or NPTX1) is a 47–50 kDa secreted glycoprotein that is expressed in the central nervous system and involved in excitatory synaptic remodeling¹.

The pentraxin superfamily members are made from monomers arranged in pentameric structures with a discoid shape. The family members are characterized by a 205 amino acids long conserved sequence located at C-terminal called the pentraxin domain with a shorter inner conserved sequence called the pentraxin signature ².

NP1 is preferentially expressed in the brain and is involved in glutamate receptor internalization. It is secreted pre-synaptically and implicated in excitatory synaptic and mitochondrial deficits. In addition, NP1 may represent a potential synapse-derived plasma biomarker that is able to identify alterations in excitatory synapses and early-onset Alzheimer's disease³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NPTX1 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (#BLP-NR191) is the original antigen used for immunization during Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191) generation. The blocking peptide binds and ‘blocks’ Anti-Neuronal Pentraxin 1 (NPTX1) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (#BLP-NR191)

Shipping and Ordering Information

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Anti-Neuronal Pentraxin 1 (NPTX1) Antibody