Name: Anti-Neuronal Pentraxin 1 (NPTX1) Antibody
Brand: Alomone Labs
SKU: ANR-191

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Overview

Cat #: ANR-191

Alternative Name Neuronal Pentraxin-1, NP1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)ERAKIESALTSLHQR, corresponding to amino acid residues 197 - 211 of mouse NPTX1 (Accession Q62443). Secreted.

Accession (Uniprot) Number Q62443

Gene ID 18164

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical; Human - 13 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):
1-2. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191), (1:400).
3-4. Anti-Neuronal Pentraxin 1 (NPTX1) Antibody, preincubated with Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (BLP-NR191).

Scientific background

Neuronal Pentraxin 1 (NP1 or NPTX1) is a 47–50 kDa secreted glycoprotein that is expressed in the central nervous system and involved in excitatory synaptic remodeling¹.

The pentraxin superfamily members are made from monomers arranged in pentameric structures with a discoid shape. The family members are characterized by a 205 amino acids long conserved sequence located at C-terminal called the pentraxin domain with a shorter inner conserved sequence called the pentraxin signature ².

NP1 is preferentially expressed in the brain and is involved in glutamate receptor internalization. It is secreted pre-synaptically and implicated in excitatory synaptic and mitochondrial deficits. In addition, NP1 may represent a potential synapse-derived plasma biomarker that is able to identify alterations in excitatory synapses and early-onset Alzheimer's disease³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (#BLP-NR191) is the original antigen used for immunization during Anti-Neuronal Pentraxin 1 (NPTX1) Antibody (#ANR-191) generation. The blocking peptide binds and ‘blocks’ Anti-Neuronal Pentraxin 1 (NPTX1) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Neuronal Pentraxin 1 (NPTX1) Blocking Peptide (#BLP-NR191)

Shipping and Ordering Information

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Anti-Neuronal Pentraxin 1 (NPTX1) Antibody