Name: Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ANR-193-F

Overview

Cat #: ANR-193-F

Alternative Name NPR, NPTXR

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RGLQDAGPRRDTMAD, corresponding to amino acid residues 162 - 176 of mouse NPTXR (Accession Q99J85). Extracellular, C-terminus.

Accession (Uniprot) Number Q99J85

Gene ID 73340

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical; human – 14 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-193), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of NPTXR by direct flow cytometry in live intact mouse J774 macrophage cell line:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC (#RIC-001-F).
^___ Cells + Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular)-FITC Antibody (#ANR-193-F), (5µg).
Cell surface detection of NPTXR by direct flow cytometry in live intact human U-87 MG glioblastoma cell line:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC (#RIC-001-F).
^___ Cells + Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular)-FITC Antibody (#ANR-193-F), (5µg).

References

Lee, S-J. et al (2017) J Neurosci, 32, 1067.
Lim, B. et al (2019), Clin Chem Lab Med., 57, 1875.
Kanda, M. et al (2020), Molec. Cancer, 19, 131.

Scientific background

Neuronal Pentraxin Receptor (NPTXR) belongs to the Pentraxin superfamily of proteins (NP) that is characterized by a pentraxin domain at the C-terminal end. It is the only pentraxin family member anchored to the cell membrane by a putative N-terminal transmembrane domain, as opposed to the other two family members, Neuronal Pentraxin1 (NP1) and Neuronal Pentraxin 2 (NP2) that are secreted proteins. NPs can form homo pentamers and hetero pentamers via their pentraxin domains¹.

NPTXR was first identified as the receptor for the snake venom toxin taipoxin and was later found to bind to AMPARs at excitatory synapses and play important, roles in the activity-dependent regulation of neural circuits via this binding activity¹.

Lately, the levels of NPTXR protein in cerebrospinal fluid has been identified as a novel potential biomarker of Alzheimer’s disease progression and could have important utility in assessing treatment success in clinical trials^2. In addition, NPTXR was identified as a molecule required for gastric cancer metastasis³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular) Antibody (#ANR-193) is a highly specific antibody directed against an extracellular epitope of the mouse NPTXR. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize NPTXR from mouse, rat and human samples.

Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular)-FITC Antibody (#ANR-193-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 18/12/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular)-FITC Antibody