Overview
- Peptide (C)REEHHFMLDARNRS, corresponding to amino acid residues 184 - 197 of rat NPFFR1 (Accession Q9EP86). Extracellular, 2nd loop.
Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (#BLP-NR073)
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:200).
3-4. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073). - Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioma cell line lysate (lanes 2 and 4):1-2. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:200).
3-4. Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073).
- Expression of NPFFR1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. NPFFR1 immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Expression of NPFFR1 in rat hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073), (1:400), followed by goat anti-rabbit-AlexaFluor-488. A. NPFFR1 immunoreactivity (green) appears in neuronal outlines (arrows) in the ventromedial hypothalamus. B. Pre-incubation of the antibody with Neuropeptide FF Receptor 1 (extracellular) Blocking Peptide (BLP-NR073), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle
- Nguyen, T. et al. (2020) J. Med. Chem. 63,21.
- Bonini, J.A. et al. (2000) J. Biol. Chem. 275,50.
- Rouméas, L. et al. (2015) Peptides. 71.
Neuropeptide FF receptor 1, NPFFR1, also known as GPR147 and RFamide-related peptide receptor 0T7T022, is a Gi/o protein-coupled receptor that mainly couples to Gαi3 and Gαs proteins and binds NPFF and RF-amide related peptides that are characterized by a conserved C-terminal RF-amide motif.1,2
NPFF has been identified in various species, including humans, rodents, bovines, and guinea pigs, indicating its physiological significance. NPFF has been detected in discrete areas of the rat central nervous system (CNS), with the highest levels observed in the dorsal spinal cord and the posterior lobe of the pituitary gland. The distribution of NPFF mRNA at the tissue level generally corresponds with NPFF immunoreactivity, except in the hypothalamic region where differences have been observed. The NPFF gene was found to be upregulated in the dorsal horn by inflammatory pain.1
NPFFR1 is expressed at a higher level than NPFFR2 in the human CNS, while NPFFR2 is more abundant than NPFFR1 in the rat brain. NPFFR1 mRNA is more abundant than NPFFR2 in the human spinal cord, whereas only NPFFR2 is found in the spinal cord of rats.1
NPFFR1 displayed a sex-biased role in food intake and metabolic homeostasis. NPFFR1 knockout male mice demonstrated reduced spontaneous food intake and suppressed leptin- and ghrelin-induced feeding. In contrast, food intake was unaltered in NPFFR1 knockout female mice. Ablation of NPFFR1 did not cause any overt alterations in body weight of male mice but increased body weight in female mice associated with increased fat mass. NPFFR1 ablation worsened glucose tolerance and insulin sensitivity caused by a high fat diet in male but not in female mice.1
NPFF has been widely recognized as an endogenous peptide that modulates the effects of opioids, with its ability to either block or potentiate morphine-induced analgesia depending on the site of administration. The increased presence of NPFF in the central nervous system (CNS) has been implicated in the development of opioid tolerance and dependence. Pharmacological blockade of NPFFR1/2 prevented the development of opioid-induced hyperalgesia and analgesic tolerance. Furthermore, the NPFF system has been shown to play a crucial role in modulating the effects of other drugs of abuse, as well as various physiological processes such as neuroendocrine and cardiovascular functions and energy homeostasis, including insulin release, food intake, memory, blood pressure, electrolyte balance, and neural regeneration. Consequently, the NPFF system has gained significant attention as a promising target for therapeutic interventions.1,3
Application key:
Species reactivity key:
Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (#ANR-073) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NPFFR1 from mouse, rat and human samples.