Name: Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-074

Overview

Cat #: ANR-074

Alternative Name NPFFR2, G-Protein Coupled Receptor 74, GPR74

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EEKYYRVRLSSHNK, corresponding to amino acid residues 186 - 199 of mouse NPFFR2 (Accession Q924H0). Extracellular, 2^nd loop.

Accession (Uniprot) Number Q924H0

Gene ID 104443

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical. Human - 12 out of 14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody
Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (#BLP-NR074)

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ip*, lci*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 3) and rat dorsal root ganglion lysate (lanes 2 and 4):
1-2. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (1:200).
3-4. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (BLP-NR074).
Western blot analysis of mouse Neuro-2a neuroblastoma cell line lysate (lanes 1 and 4), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
1-3. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (1:200).
4-6. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (BLP-NR074).

Indirect flow cytometry

Cell surface detection of NPFFR2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (2.5μg) + goat-anti-rabbit-FITC.

References

Quillet, R. et al. (2016) Pharmacol. Ther. 160, 84.
Lin, Y-T. et al. (2019) Chin. J. Physiol. 62, 47.
Koller, J. et al. (2021) Neuropeptides. 90, 102198.
Nguyen, T. et al. (2020) J. Med. Chem. 63, 12387.
Lin, Y-T. et al. (2016) Eur. J. Pain. 20, 895.
Zhang, L. et al. (2016) Eur. J. Pain. 20, 895.
Hassnain Waqas, S.F. et al. (2017) J. Clin. Invest. 127, 2842.

Scientific background

Neuropeptide FF receptor 2, NPFFR1, and also known as GPR74, is a member of the G protein-coupled receptor superfamily containing two subtypes, NPFFR1 and NPFFR2.^1-2

NPFFR1 and NPFFR2 are the high affinity receptors the endogenous neuropeptides NPFF and NPAF, also known as RFamide peptides, which are characterized by their Arg-Phe-NH2 motif at the C-terminal end.^1-2

NPFFR2 is expressed in both the central and peripheral nervous system, as well as in other organs such as heart, kidney and adipose tissue macrophages.^1-4

NPFFR2 has been extensively studied for its role in pain modulation. Mice over-expressing NPFFR2 showed more sensitivity to mechanical and thermal noxious stimuli. In addition, a role in the modulation of the development of opioid tolerance and the regulation of withdrawal symptoms, has been suggested.^3,5

NPFFR2 signaling has been implicated in the regulation of energy homeostasis, through the inhibition of food intake and the modulation of metabolic rate and energy expenditure.^2,3,6 In this regard, it is interesting to note that, NPFFR2 has been identified in adipose tissue macrophages, cells that are involved in the development of obesity-induced metabolic diseases.⁷

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize NPFFR2 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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