Name: Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-074

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Overview

Cat #: ANR-074

Alternative Name NPFFR2, G-Protein Coupled Receptor 74, GPR74

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EEKYYRVRLSSHNK, corresponding to amino acid residues 186 - 199 of mouse NPFFR2 (Accession Q924H0). Extracellular, 2^nd loop.

Accession (Uniprot) Number Q924H0

Gene ID 104443

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical. Human - 12 out of 14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody
Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (#BLP-NR074)

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ip*, lci*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 3) and rat dorsal root ganglion lysate (lanes 2 and 4):
1-2. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (1:200).
3-4. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (BLP-NR074).
Western blot analysis of mouse Neuro-2a neuroblastoma cell line lysate (lanes 1 and 4), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
1-3. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (1:200).
4-6. Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody, preincubated with Neuropeptide FF Receptor 2 (extracellular) Blocking Peptide (BLP-NR074).

Indirect flow cytometry

Cell surface detection of NPFFR2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (#ANR-074), (2.5μg) + goat-anti-rabbit-FITC.

Scientific background

Neuropeptide FF receptor 2, NPFFR1, and also known as GPR74, is a member of the G protein-coupled receptor superfamily containing two subtypes, NPFFR1 and NPFFR2.^1-2

NPFFR1 and NPFFR2 are the high affinity receptors the endogenous neuropeptides NPFF and NPAF, also known as RFamide peptides, which are characterized by their Arg-Phe-NH2 motif at the C-terminal end.^1-2

NPFFR2 is expressed in both the central and peripheral nervous system, as well as in other organs such as heart, kidney and adipose tissue macrophages.^1-4

NPFFR2 has been extensively studied for its role in pain modulation. Mice over-expressing NPFFR2 showed more sensitivity to mechanical and thermal noxious stimuli. In addition, a role in the modulation of the development of opioid tolerance and the regulation of withdrawal symptoms, has been suggested.^3,5

NPFFR2 signaling has been implicated in the regulation of energy homeostasis, through the inhibition of food intake and the modulation of metabolic rate and energy expenditure.^2,3,6 In this regard, it is interesting to note that, NPFFR2 has been identified in adipose tissue macrophages, cells that are involved in the development of obesity-induced metabolic diseases.⁷

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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