Overview
- Peptide CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2 (Accession Q63384). Intracellular, C-terminus.
Western blot analysis of rat dorsal root ganglion lysates:1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Western blot analysis of rat brain lysates:1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Western blot analysis of mouse brain lysates:1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:400).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Expression of NTR2 in rat mediobasal hypothalamusImmunohistochemical staining of perfusion-fixed brain frozen rat mediobasal (MB) hypothalamus sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:100), (green). A. NTR2 appears in neuronal fibers marked by white arrows. B. In the same section, there is staining for glial fibrillary acidic protein (red fluorescence), a marker of astrocytes. C. Merging the two images demonstrates that NTR2 is not localized in astrocytes. DAPI is used as the counterstain (blue).
Expression of NTR2 in rat stomachImmunohistochemical staining of paraffin-embedded rat stomach sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:50), (brown). Staining is specific for parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
Expression of NTR2 in rat C6 glioma cellsImmunocytochemical staining of rat C6 glioma cells. A. Paraformaldehyde fixed and permeabilized rat C6 glioma cells were stained with Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200), followed by Alexa-555 conjugated goat-anti-rabbit- secondary antibody (orange staining). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field as in (A).
- Vincent, J.P. et al. (1999) Trends Pharmacol. Sci. 20, 302.
- Schulz, S. et al. (2006) J. Endocrinol. 191, 121.
- Maeno, H. et al. (2004) Brain Res. 998, 122.
Neurotensin receptor 2 (NTR2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.
NTR2 and NTR1 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1
NTR2 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+mobilization. However, the exact signaling mechanisms subsequent to NTR2 activation appear to depend on the species and on the cellular system in which the receptor is studied.
NTR2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acid secretion.2
The most established physiological role of NTR2 is its role in pain transmission as NTR2-defficient mice showed that NTR2 is the likely mediator of the neurotensin-induced analgesic responses.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope at the intracellular C-terminal domain of the rat Neurotensin receptor type 2. Anti-Neurotensin Receptor 2 Antibody (#ANT-016) can be used in western blot, immunohistochemical and immunocytochemical applications, and recognizes NTR2 from rat and mouse samples.
Applications
Citations
Powered by Bioz- Saada, S. et al. (2012) J. Immunol. 189, 5293.
