Name: Anti-Neurotensin Receptor 2 Antibody
Brand: Alomone Labs
SKU: ANT-016

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Overview

Cat #: ANT-016

Alternative Name NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2 (Accession Q63384). Intracellular, C-terminus.

Accession (Uniprot) Number Q63384

Gene ID 64636

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 14/16 amino acid residues identical.

RRID AB_2040040.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Unlikely to recognize human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Rat and mouse brain and dorsal root ganglion lysates (1:200-1:400).
Western blot analysis of rat dorsal root ganglion lysates:
1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Western blot analysis of rat brain lysates:
1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Western blot analysis of mouse brain lysates:
1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:400).
2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).

Immunohistochemistry

Rat dorsal root ganglion sections (frozen), (1:100) and stomach sections (paraffin embedded), (1:50).

Immunocytochemistry

Paraformaldehyde-fixed rat C6 glioma cells (1:200).

Scientific background

Neurotensin receptor 2 (NTR2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.

NTR2 and NTR1 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.¹

NTR2 signals preferentially through Ga_q, resulting in the activation phopholipase C and intracellular Ca²⁺mobilization. However, the exact signaling mechanisms subsequent to NTR2 activation appear to depend on the species and on the cellular system in which the receptor is studied.

NTR2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acid secretion.²

The most established physiological role of NTR2 is its role in pain transmission as NTR2-defficient mice showed that NTR2 is the likely mediator of the neurotensin-induced analgesic responses.³

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016) is the original antigen used for immunization during Anti-Neurotensin Receptor 2 Antibody (#ANT-016) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotensin Receptor 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016)

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Certificate of Analysis

Anti-Neurotensin Receptor 2 Antibody