Name: Anti-NGL-2/LRRC4 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-162

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Overview

Cat #: ANR-162

Alternative Name Netrin-G2 Ligand, Brain Tumor-Associated Protein BAG, Leucine-Rich Repeat-Containing Protein 4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)STDTTDKMQTSLDE, corresponding to amino acid residues 507 - 520 of mouse LRRC4 (Accession Q99PH1). Extracellular, N-terminus.

Accession (Uniprot) Number Q99PH1

Gene ID 192198

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical, Human 13 out of 14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1-2. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200).
3-4. Anti-NGL-2/LRRC4 (extracellular) Antibody, preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysates:
1. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200).
2. Anti-NGL-2/LRRC4 (extracellular) Antibody, preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).

Indirect flow cytometry

Cell surface detection of NGL-2/LRRC4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (2.5μg) + goat-anti-rabbit-APC.

Scientific background

The leucine-rich repeat (LRR) superfamily is a diverse protein family that consist of leucine-rich domains (LRR domains). The LRR domain is known to regulate protein-protein interaction or cell adhesion. Large portion of LRR proteins mediate the differentiation and development of nervous tissues¹.

One member of the LRR superfamily is leucine rich repeat containing protein 4 (LRRC4), also known as netrin-G ligand-2 (NGL-2), and serves as a receptor for netrin-G2 (NTNG2). In addition to the LRR domain LRRC4 consist of immunoglobulin (Ig) domain in the extracellular region, a transmembrane domain, and a cytoplasmic region that terminates in a protein-protein interaction domain (PDZ)-binding motif. LRRC4 regulates the formation of excitatory synapses and promotes axon differentiation².

Functionally, deletion of LRRC4 in mice was found to suppress excitatory synapse development and synaptic transmission. In addition, LRRC4 gene has been identified as tumor suppressor³. In human it has been implicated in autism spectrum disorder (ASD), schizophrenia, and intellectual disability. Moreover, netrin-G2, the presynaptic ligand of LRRC4, is linked to schizophrenia and bipolar disorder^4-5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

NGL-2/LRRC4 (extracellular) Blocking Peptide (#BLP-NR162) is the original antigen used for immunization during Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162) generation. The blocking peptide binds and ‘blocks’ Anti-NGL-2/LRRC4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

NGL-2/LRRC4 (extracellular) Blocking Peptide (#BLP-NR162)

Shipping and Ordering Information

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Certificate of Analysis

Anti-NGL-2/LRRC4 (extracellular) Antibody