Overview
- Peptide (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3 (Accession P0C192). Extracellular, N-terminus.
Western blot analysis of rat new born brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
3,4. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163).
Western blot analysis of mouse brain lysate:1. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
2. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163).
Expression of NGL-3 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NGL-3 immunoreactivity (green) is detected in the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
- Seiradake, E. et al. (2011) EMBO J. 30, 4479.
- Kwon, S.K. et al. (2010) Biol. Chem. 285, 13966.
- Lin, J.C. et al. (2003) Neurosci. 6, 1270.
- Kim, S. et al. (2006) Neurosci. 9, 1294.
- Woo, J. et al. (2009) Neurosci. 12, 428.
- Lee, H. et al. (2019) PLoS Biol. 17, e2005326.
NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.
The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.
NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.
Mice lacking NGL-3 (Ngl3−/−) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.
NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development6.
Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.
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