Name: Anti-NHERF-1/EBP50 Antibody
Brand: Alomone Labs
SKU: APZ-006

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Overview

Cat #: APZ-006

Alternative Name Na⁺/H⁺ exchange regulatory cofactor 1, Ezrin-radixin-moesin-binding phosphoprotein 50, SLC9A3R1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: r

Immunogen

Peptide (C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1 (Accession Q9JJ19). Between the PDZ2 and the ERM binding domains.

Accession (Uniprot) Number Q9JJ19

Gene ID 59114

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.

RRID AB_2039834.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.2 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

Western blot

Rat kidney membranes (1:200-1:500).
Western blotting of rat kidney membranes:
1. Anti-NHERF-1/EBP50 Antibody (APZ-006), (1:200).
2. Anti-NHERF-1/EBP50 Antibody, preincubated with NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006).

Scientific background

The Na⁺/H⁺ exchange regulatory factor (EBP-50 or NHERF-1) is a 55 kD cytoplasmic protein adaptor that recruits a wide variety of cellular proteins.

Many of the interacting proteins do so through the two tandem PDZ domains (protein-binding domains conserved in the mammalian synaptic protein PSD-95/DlgA/ZO-1) and the C-terminal ERM (ezrin, radixin, moesin) binding region.

NHERF-1 was first identified as an adaptor necessary for the function of the Na⁺/H⁺exchanger isoform 3 (NHE3) in renal apical cells.¹ Since then it has been identified in cells of epithelial origin in several tissues such as gastrointestinal and lung.

NHERF-1 has been shown to interact with a growing number of proteins including ion channels (ROMK, CFTR, P2Y1, TRPC4 and TRPC6), growth factor receptors (PDGFR), phospholipase C isoforms (PLCb1, PLCb2, PLCb3), non-receptor protein tyrosine kinases (YAP65) and several cytoskeletal proteins that link membrane proteins to the underlying actin cytoskeleton.²

Recently, it has been shown that NHERF-1 expression was elevated in breast tumors compared to the expression in adjacent normal tissue.³

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006) is the original antigen used for immunization during Anti-NHERF-1/EBP50 Antibody (#APZ-006) generation. The blocking peptide binds and ‘blocks’ Anti-NHERF-1/EBP50 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

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Certificate of Analysis

Anti-NHERF-1/EBP50 Antibody