Name: Anti-NMUR1 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-071

Overview

Cat #: ANR-071

Alternative Name Neuromedin-U Receptor 1, G Protein-Coupled Receptor 66, GPR66

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DRLMWSMVSHWTDGLR, corresponding to amino acid residues 316 - 331 of rat NMUR1 (Accession Q9JJI5). Extracellular, 3^rd loop.

Accession (Uniprot) Number Q9JJI5

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human – 12 out of 16 amino acid residues identical;
Mouse - 10 out of 16 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1-2. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:500).
3-4. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).
Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human K562 myelogenous leukemia cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8):
1-4. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:400).
5-8. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).

Immunohistochemistry

Expression of NMUR1 in rat cerebellum.
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1: 300), followed by goat anti-rabbit-AlexaFluor-488. A. NMUR1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with NMUR1 (extracellular) Blocking Peptide (BLP-NR071), suppressed staining. Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Cell surface detection of NMUR1 by indirect flow cytometry in live intact mouse J774 macrophage cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-NMUR1 (extracellular) Antibody (#ANR-071), (2.5μg) + goat-anti-rabbit-FITC.

References

Brighton, P.J. et al. (2004) Pharmacol. Rev. 56, 231.
Panetta, R. et al (2013) Physiol Genomics. 45, 89.

Scientific background

Neuromedin U Receptor 1 (NMUR1) is one of the two receptors for the neuropeptide Neuromedin U (NMU). NMUR1 and the second receptor NMUR2 are members of the G protein coupled receptors (GPCRs) superfamily ¹.

NMU is a neuropeptide with potent activity on smooth muscles that is widely distributed in the gut and central nervous system. NMU interaction with one of its two receptors- NMU1R or NMU2R, mediates the stimulation of smooth muscles, increase of blood pressure, alteration of ion transport in the gut, control of local blood flow and regulation of adrenocortical function^1-2.

NMU1R is abundantly expressed in peripheral tissues including lung, GI tract, pancreatic islets, testis, adipose, immune tissues, and dorsal root ganglia², and due to its diverse functions it is a very appealing drug and research candidate.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-NMUR1 (extracellular) Antibody (#ANR-071) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NMUR1 from rat, mouse and human samples

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

NMUR1 (extracellular) Blocking Peptide (#BLP-NR071) is the original antigen used for immunization during Anti-NMUR1 (extracellular) Antibody (#ANR-071) generation. The blocking peptide binds and ‘blocks’ Anti-NMUR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

NMUR1 (extracellular) Blocking Peptide (#BLP-NR071)

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Anti-NMUR1 (extracellular) Antibody