Name: Anti-NMUR1 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-071

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Overview

Cat #: ANR-071

Alternative Name Neuromedin-U Receptor 1, G Protein-Coupled Receptor 66, GPR66

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DRLMWSMVSHWTDGLR, corresponding to amino acid residues 316 - 331 of rat NMUR1 (Accession Q9JJI5). Extracellular, 3^rd loop.

Accession (Uniprot) Number Q9JJI5

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human – 12 out of 16 amino acid residues identical;
Mouse - 10 out of 16 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1-2. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:500).
3-4. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).
Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human K562 myelogenous leukemia cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8):
1-4. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:400).
5-8. Anti-NMUR1 (extracellular) Antibody, preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).

Scientific background

Neuromedin U Receptor 1 (NMUR1) is one of the two receptors for the neuropeptide Neuromedin U (NMU). NMUR1 and the second receptor NMUR2 are members of the G protein coupled receptors (GPCRs) superfamily ¹.

NMU is a neuropeptide with potent activity on smooth muscles that is widely distributed in the gut and central nervous system. NMU interaction with one of its two receptors- NMU1R or NMU2R, mediates the stimulation of smooth muscles, increase of blood pressure, alteration of ion transport in the gut, control of local blood flow and regulation of adrenocortical function^1-2.

NMU1R is abundantly expressed in peripheral tissues including lung, GI tract, pancreatic islets, testis, adipose, immune tissues, and dorsal root ganglia², and due to its diverse functions it is a very appealing drug and research candidate.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

NMUR1 (extracellular) Blocking Peptide (#BLP-NR071) is the original antigen used for immunization during Anti-NMUR1 (extracellular) Antibody (#ANR-071) generation. The blocking peptide binds and ‘blocks’ Anti-NMUR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

NMUR1 (extracellular) Blocking Peptide (#BLP-NR071)

Shipping and Ordering Information

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Certificate of Analysis

Anti-NMUR1 (extracellular) Antibody