Overview
- Peptide (C)ESRRVSGRTYGAAR, corresponding to amino acid residues 145 - 158 of mouse NPBWR1 (Accession P49681). Intracellular, 2nd loop.
NPBWR1 Blocking Peptide (#BLP-NR075)
Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysates (lanes 2 and 5) and rat heart lysate (lanes 3 and 6):1-3. Anti-NPBWR1 Antibody (#ANR-075), (1:400).
4-6. Anti-NPBWR1 Antibody, preincubated with NPBWR1 Blocking Peptide (BLP-NR075).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (lanes 2 and 4):1-2. Anti-NPBWR1 Antibody (#ANR-075), (1:400).
3-4. Anti-NPBWR1 Antibody, preincubated with NPBWR1 Blocking Peptide (BLP-NR075).
Expression of NPBWR1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NPBWR1 Antibody (#ANR-075), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NPBWR1 immunoreactivity (green) appears in the mossy fiber terminal zone (MFT, arrows). B. Pre-incubation of the antibody with NPBWR1 Blocking Peptide (BLP-NR075), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Sakurai, T. (2013) Front. Endocrinol. 4, 23.
- Nguyen, T. et al. (2022) Eur. J. Med. Chem. 231, 114149.
- Wojciechowicz, T. et al. (2021) Int. J. Mol. Sci. 22, 6632.
- Xing, Y. et al. (2021) Eur. J. Pharmacol. 898, 173979.
Neuropeptides B/W receptor type 1 (NPBWR1), also known as G-protein coupled receptor 7 (GPR7), is a member of the G-protein coupled receptor (GPCR) superfamily.
NPBWR1 mediates the biological activities of Neuropeptide B (NPB) and neuropeptide W (NPW) that are structurally and functionally related endogenous neuropeptides. In humans, a second receptor termed NPBWR2 or GPR8, has been identified, however, NPBWR2 is absent in rodents.1,2
NPBWR1 is expressed in the central nervous system (CNS) and in peripheral tissues. In the brain expression of NPBWR1 is found in several regions including hypothalamus, hippocampus and amygdala. Indeed, the distribution of NPBWR1 and its ligands in brain is in accordance to their established functions in appetite control and feeding behavior, as well as locomotion, analgesia and stress response.2,3
Several studies have pointed to the role of NPBWR1 in pain modulation. Interestingly, NPBWR1 has structural similarities with the opioid receptors and in fact, it binds non-selective opioid ligands such as β-endorphin.3 It has been suggested, that a combination therapies of morphine (an opioid receptor ligand) and NPBWR1 agonists could provide a new avenue to treat inflammatory and neuropathic pain with less risk of adverse effects. 4
In the periphery, NPBWR1 may be involved in energy homeostasis by regulating insulin secretion from pancreatic β cells and regulating white and brown adipose tissue. 3
Anti-NPBWR1 Antibody (#ANR-075) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NPBWR1 from rat, mouse and human samples.
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