Overview
- Peptide CFDKFPSDSHRLSY, corresponding to amino acid residues 197-210 of mouse NPY1R (Accession Q04573). 2nd extracellular loop.
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200).
3, 4. Anti-NPY1R (extracellular) Antibody, preincubated with NPY1R (extracellular) Blocking Peptide (#BLP-NR027).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):1, 2. Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200).
3, 4. Anti-NPY1R (extracellular) Antibody, preincubated with NPY1R (extracellular) Blocking Peptide (#BLP-NR027).
Expression of NPY1R in rat striatum and subventricular zone (SVZ)Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NPY1R (extracellular) Antibody (#ANR-027), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NPY1R immunoreactivity (green) appears in the striatum (STR) and the SVZ. Cell nuclei were stained with DAPI (blue). LV = Lateral ventricle.
Cell surface detection of NPY1R in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-NPY1R (extracellular) Antibody (#ANR-027), (2.5 µg) + goat-anti-rabbit-FITC.
- Yang, Z. et al. (2018) Nature 556, 520.
- Comeras, L.B. et al. (2019) Ann. N.Y. Acad. Sci. 1455, 59.
- Fetissov, S.O. et al. (2004) Neuropeptides 38, 175.
- Dumont, Y. et al. (1998) J. Comp. Neurol. 402, 372.
- Holzer, P. et al. (2012) Neuropeptides 46, 261.
Neuropeptide Y1 receptor (NPY1R) is one out of four receptors (NPY1R, NPY2R, NPY4R and NPY5R) that bind neuropeptide Y (NPY), an abundant neuropeptide in the mammalian central and peripheral nervous systems.
NPY1R is part of the β-branch of class A G-protein coupled receptors and likewise, has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1,2.
NPY belongs to a family of gut-brain peptides that demonstrate both hormonal functions by paracrine and endocrine release in the gastrointestinal tract and a neurotransmitter functions in sympathetic ganglia and the CNS. It is highly expressed throughout the brain with particularly high expression in limbic brain areas, such as the hippocampus, amygdala, and hypothalamus3,4. NPY modulates multiple physiological and pathophysiological processes: it increases food intake and reduces fear and anxiety by activating NPY1R1,2.
Because of the multiple functions of NPY, NPY1R has great importance in the development and progression of both metabolic and neurological diseases such as diabetes, obesity, breast cancer and bone loss and indeed a number of peptides and small-molecule compounds have been characterized as NPY1R antagonists and have shown clinical potential in the treatment of these pathological states5.
Anti-NPY1R (extracellular) Antibody (#ANR-027) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NPY1R from mouse, rat, and human samples.
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