Name: Anti-NrCAM (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ANR-045-F

Overview

Cat #: ANR-045-F

Alternative Name Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM (Accession P97686). Extracellular, N-terminus.

Accession (Uniprot) Number P97686

Homology Mouse – 15/16 amino acid residues identical; human – 13/16 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-045), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of NrCAM by direct flow cytometry in live intact mouse P815 mastocytoma cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-NrCAM (extracellular)-FITC Antibody (#ANR-045-F), (2.5µg).
Cell surface detection of NrCAM by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-NrCAM (extracellular)-FITC Antibody (#ANR-045-F), (5µg).

References

Mohan, V. et al. (2019) Front. Cell Dev. Biol. 7, 9.
Brummer, T. et al. (2019) EMBO Mol. Med. 11, 4.
Demyanenko, G.P. et al. (2014) J. Neurosci. 34, 11274.

Scientific background

NrCAM (Neuron-glia related cell adhesion molecule) is a cell adhesion molecule that belongs to the immunoglobulin-class (Ig) of the L1 family. NrCAM promotes axon growth and repulsion through trans homophilic binding of extracellular domains and intracellular coupling of the conserved cytoplasmic domains to the actin cytoskeleton. This interaction occurs through ankyrin B and ezrin-radixin-moesin adaptors. In addition, NrCAM is responsible for controlling dendritic spine densities, axonal guidance and targeting, and neurite outgrowth by acting as a co‐receptor molecule at the neuronal cell surface^1,2.

NrCAM is highly expressed in the cortex^1,3. Its structure contains a PDZ binding motif with the potential to bind PSD scaffold proteins PSD-95 and SAP102. The Ig domains of the protein mediate homophilic and heterophilic binding to multiple ligands^2,3.

Genetic polymorphisms in the NrCAM gene are linked to autism spectrum disorders, intellectual disability and addictive behavior. NrCAM knockout mice demonstrate autism spectrum disorder related behaviors including impaired sociability, cognitive inflexibility, hypersensitivity to sensory stimuli and altered visual cortical responses.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-NrCAM (extracellular) Antibody (#ANR-045) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NrCAM from mouse, rat and human samples.

Anti-NrCAM (extracellular)-FITC Antibody (#ANR-045-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NrCAM from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-NrCAM (extracellular)-FITC Antibody