Overview
- Peptide LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4) (Accession P34130).
- 10 ng Recombinant human Neurotrophin-4 (NT-4) protein (1:200). Do not use milk for blocking with this antibody.
Western blot analysis of Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (10 ng):1. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004), (1:200).
2. Anti-Neurotrophin 4 (NT-4) Antibody, preincubated with Neurotrophin 4/NT-4 Blocking Peptide (#BLP-NT004).
- Mouse spinal cord.
The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is synthesized by keratinocytes.4 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.5
The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6 to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9
The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for NT-4 and BDNF, and p75NTR, which binds all the neurotrophins.10
