Overview
- Peptide GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor) (Accession P20783).
Western blot analysis of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (100 ng):1. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:200)2. Anti-Neurotrophin 3 (NT-3) Antibody, preincubated with Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003).
Expression of Neurotrophin 3 in mouse spinal cordImmunohistochemical staining of perfusion-fixed frozen mouse spinal cord sections with Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 (green). The same sections were stained with mouse anti-neurofilament 200 (NF) (red). Merge of the two images demonstrates the presence of NT-3 in gray matter which colocalizes with NF only in the soma of some neurons (arrow).- Rat hippocampus (1:300) (Ramos Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248).
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The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.2 The rat and human forms of neurotrophin-3 (NT-3) are 96% homologous.
NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3 to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75NTR, which binds all the neurotrophins.7
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Neurotrophin 3. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003) can be used in western blot and immunohistochemical applications. It has been designed to recognize NT-3 from rat, mouse and human samples.
Applications
Citations
Powered by Bioz- Mouse spinal cord sections (1:200).
Kumar, H. et al. (2020) J. Neurosci. 40, 1943. - Rat hippocampus (1:300).
Ramos-Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248.
- Mouse spinal cord sections (1:200).
Kumar, H. et al. (2020) J. Neurosci. 40, 1943.
