Name: Anti-Neurotrophin 3 (NT-3) Antibody
Brand: Alomone Labs
SKU: ANT-003

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Overview

Cat #: ANT-003

Alternative Name NTF3, Nerve growth factor 2, NGF2, HDNF

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor) (Accession P20783).

Accession (Uniprot) Number P20783

Gene ID 4908

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse, chicken, Xenopus - identical; cat - 9/10 amino acid residues identical.

RRID AB_2040013.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.3 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

10 ng Recombinant human Neurotrophin-3 (NT-3) protein (1:1000).
Western blot analysis of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260), (100 ng):
1. Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003), (1:200)

2. Anti-Neurotrophin 3 (NT-3) Antibody, preincubated with Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003).

Immunohistochemistry

Mouse spinal cord. Rat hippocampus (1:300) (Ramos Languren, L.E. and Escobar, M.L. (2013) Eur. J. Neurosci. 37, 1248).

Scientific background

The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.¹ The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.² The rat and human forms of neurotrophin-3 (NT-3) are 96% homologous.

NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,³ to serve as an anti-inflammatory factor, to suppress microglial activation,⁴ to play a critical role in regulating T helper 1/T helper 2 cell balance⁵ and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.⁶ The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75^NTR, which binds all the neurotrophins.⁷

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003) is the original antigen used for immunization during Anti-Neurotrophin 3 (NT-3) Antibody (#ANT-003) generation. The blocking peptide binds and ‘blocks’ Anti-Neurotrophin 3/NT-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Neurotrophin 3/NT-3 Blocking Peptide (#BLP-NT003)

Shipping and Ordering Information

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Anti-Neurotrophin 3 (NT-3) Antibody