Name: Anti-OCTN1/SLC22A4 Antibody
Brand: Alomone Labs
SKU: ACT-014

Overview

Cat #: ACT-014

Alternative Name Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EQMQKVRGFR(S)GKKS, corresponding to amino acid residues 522 - 536 of mouse OCTN1 (Accession Q9Z306). Intracellular, C-terminus.

Accession (Uniprot) Number Q9Z306

Gene ID 30805

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 14 out of 15 amino acid residues identical; Human – 12 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat small intestine lysates (lanes 2 and 4):
1-2. Anti-OCTN1/SLC22A4 Antibody (#ACT-014), (1:200).
3-4. Anti-OCTN1/SLC22A4 Antibody, preincubated with
OCTN1/SLC22A4 Antibody Blocking Peptide (BLP-CT014).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1-2. Anti-OCTN1/SLC22A4 Antibody (#ACT-014), (1:600).
3-4. Anti-OCTN1/SLC22A4 Antibody, preincubated with
OCTN1/SLC22A4 Antibody Blocking Peptide (BLP-CT014).
Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 4), mouse BV-2 microglia cell line lysates (lanes 2 and 5) and human HeLa cervix adenocarcinoma cell line lysates (lanes 3 and 6):
1-3. Anti-OCTN1/SLC22A4 Antibody (#ACT-014), (1:400).
4-6. Anti-OCTN1/SLC22A4 Antibody, preincubated with
OCTN1/SLC22A4 Antibody Blocking Peptide (BLP-CT014).

Immunohistochemistry

Expression of OCTN1 in rat hippocampus.
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-OCTN1/SLC22A4 Antibody (#ACT-014), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. OCTN1 immunoreactivity (green) appears in neuronal profiles in the pyramidal layer (P, arrows). B. Pre-incubation of the antibody with OCTN1/SLC22A4 Antibody Blocking Peptide (BLP-CT014), suppressed staining. Cell nuclei are stained with DAPI (blue).

References

Nakamichi, N. et al (2017) Biol. Pharm. Bull, 40, 1146.
Pochini, L. et al (2019) SLAS Discov, 24, 89.
Grundemann, D. et al (2005) PNAS, 102, 5256.

Scientific background

Organic Cation Transporter Novel (OCTN) is a subfamily of the larger Organic Cation Transporter (OCT) or SLC22 transporter family.

The OCTN subfamily consists of two members in humans: OCTN1 (SLC22A4) and OCTN2 (SLC22A5) with a third member (SLC22A21) present only in rodents ^1-2.

The physiological role of OCTN1 (SLC22A4) has not yet been elucidated. OCTN1 is ubiquitously expressed throughout the body and transports various therapeutic agents, including organic cations and zwitterions. Ergothioneine (ET/ ERGO) a natural diet-derived antioxidant was found to be a specific substrate of OCTN1 ³.

OCTN1 is functionally expressed in neurons and neural stem cells and activation of the transporter could conceivably have a protective role in neurodegenerative diseases. In addition, in peripheral organs, mutations in OCTN1 are associated with susceptibility to Crohn’s disease and rheumatoid arthritis suggesting that OCTN1 may play a role in inflammatory dieseases ¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-OCTN1/SLC22A4 Antibody (#ACT-014) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize OCTN1 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

OCTN1/SLC22A4 Blocking Peptide (#BLP-CT014) is the original antigen used for immunization during Anti-OCTN1/SLC22A4 Antibody (#ACT-014) generation. The blocking peptide binds and ‘blocks’ Anti-OCTN1/SLC22A4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

OCTN1/SLC22A4 Blocking Peptide (#BLP-CT014)

Shipping and Ordering Information

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Anti-OCTN1/SLC22A4 Antibody