Name: Anti-OCTN1/SLC22A4 Antibody
Brand: Alomone Labs
SKU: ACT-014

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Overview

Cat #: ACT-014

Alternative Name Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EQMQKVRGFR(S)GKKS, corresponding to amino acid residues 522 - 536 of mouse OCTN1 (Accession Q9Z306). Intracellular, C-terminus.

Accession (Uniprot) Number Q9Z306

Gene ID 30805

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 14 out of 15 amino acid residues identical; Human – 12 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat small intestine lysates (lanes 2 and 4):
1-2. Anti-OCTN1/SLC22A4 Antibody (#ACT-014), (1:200).
3-4. Anti-OCTN1/SLC22A4 Antibody, preincubated with
OCTN1/SLC22A4 Antibody Blocking Peptide (BLP-CT014).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1-2. Anti-OCTN1/SLC22A4 Antibody (#ACT-014), (1:600).
3-4. Anti-OCTN1/SLC22A4 Antibody, preincubated with
OCTN1/SLC22A4 Antibody Blocking Peptide (BLP-CT014).
Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 4), mouse BV-2 microglia cell line lysates (lanes 2 and 5) and human HeLa cervix adenocarcinoma cell line lysates (lanes 3 and 6):
1-3. Anti-OCTN1/SLC22A4 Antibody (#ACT-014), (1:400).
4-6. Anti-OCTN1/SLC22A4 Antibody, preincubated with
OCTN1/SLC22A4 Antibody Blocking Peptide (BLP-CT014).

Scientific background

Organic Cation Transporter Novel (OCTN) is a subfamily of the larger Organic Cation Transporter (OCT) or SLC22 transporter family.

The OCTN subfamily consists of two members in humans: OCTN1 (SLC22A4) and OCTN2 (SLC22A5) with a third member (SLC22A21) present only in rodents ^1-2.

The physiological role of OCTN1 (SLC22A4) has not yet been elucidated. OCTN1 is ubiquitously expressed throughout the body and transports various therapeutic agents, including organic cations and zwitterions. Ergothioneine (ET/ ERGO) a natural diet-derived antioxidant was found to be a specific substrate of OCTN1 ³.

OCTN1 is functionally expressed in neurons and neural stem cells and activation of the transporter could conceivably have a protective role in neurodegenerative diseases. In addition, in peripheral organs, mutations in OCTN1 are associated with susceptibility to Crohn’s disease and rheumatoid arthritis suggesting that OCTN1 may play a role in inflammatory dieseases ¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

OCTN1/SLC22A4 Blocking Peptide (#BLP-CT014) is the original antigen used for immunization during Anti-OCTN1/SLC22A4 Antibody (#ACT-014) generation. The blocking peptide binds and ‘blocks’ Anti-OCTN1/SLC22A4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

OCTN1/SLC22A4 Blocking Peptide (#BLP-CT014)

Shipping and Ordering Information

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Certificate of Analysis

Anti-OCTN1/SLC22A4 Antibody