Overview
- Peptide (C)DIHGLG(S)TVDWRSKD, corresponding to amino acid residues 180 - 194 of mouse Opsin 3 (Accession Q9WUK7). Extracellular, 2nd loop.
1 µg peptide per 1 µg antibody
Opsin 3 (extracellular) Blocking Peptide (#BLP-OR023)
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:200).
3-4. Anti-Opsin 3 (extracellular) Antibody, preincubated with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023).
Western blot analysis of rat eye lysate:1. Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:200).
2. Anti-Opsin 3 (extracellular) Antibody, preincubated with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023).
Western blot analysis of Malme-3 skin fibroblast cell line lysates (lanes 1 and 5), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 6), human Jurkat T-cell leukemia cell line lysate (lanes 3 and 7) and human ARPE-19 retinal pigmental epithelia cell line lysate (lanes 4 and 8):1-4. Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:200).
5-8. Anti-Opsin 3 (extracellular) Antibody, preincubated with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023).
Expression of Opsin 3 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the CA3 region, showed Opsin 3 immunoreactivity (green), in the pyramidal layer (P, left pointing arrows) and in the mossy fiber terminal zone (MFT, right pointing arrows). B. Pre-incubation of the antibody with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Opsin 3 in rat optic tract.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Opsin 3 immunoreactivity (green), appears in the optic tract (OT, arrows). B. Pre-incubation of the antibody with Opsin 3 (extracellular) Blocking Peptide (BLP-OR023), suppressed staining. Cell nuclei are stained with DAPI (blue).
Cell surface detection of Opsin 3 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:___ Cells.
___ Cells + goat-anti-rabbit-PE.
___ Cells + Anti-Opsin 3 (extracellular) Antibody (#AOR-023), (2.5μg) + goat-anti-rabbit-PE.
- Leung, N.Y. et al. (2018) Ann. Rev. Cell Dev. Biol. 33, 241.
- Andrabi, M. et al. (2023) Ann. Rev. Vis. Sci. 9, 245.
- Moraes, M.N. et al. (2021) Cell.Tissue Res. 385, 519.
- Olinski, L.E. et al. (2020) Ad. Bio. Regul. 75, 100668.
- Nayak, G. et al. (2020) Cell Rep. 30, 672.
- Olinski, L.E. et al. (2020) eNeuro. 7, ENEURO.0107-20.2020.
- White, J.H. et al. (2008) Hum. Mol. Gen. 17, 1890.
Opsin 3 (OPN3), also known as encephalopsin or panopsin, is a member of the opsin family, which are light-sensitive G protein–coupled receptors (GPCRs) primarily known for their role in vision.1-3
Opsins are found throughout the animal kingdom and while most vertebrates have a similar repertoire of 6–20 genes, mammals most commonly have eight opsins, although humans have nine.1-3
Unlike Rhodopsin (or Opsin 2), the founding member of the opsin family, which is located in the retina and is essential for vision in low-light conditions, Opsin 3 has a more widespread expression pattern and functions that extend beyond traditional visual processing.1-3
Opsin 3 is expressed in various tissues, including the brain, skin, testes, and lungs, among others.1-3
Opsin 3 is abundantly expressed in the skin (a major light sensing organ). The function of Opsin 3 in photodetection and photosensitivity is not clear, but it appears to be based in a light-independent manner and through the interaction with MC1R, another GPCR that is crucial for the regulation of pigmentation in the skin and hair. 4
An intriguing role for Opsin 3 was described in relation to regulation of energy metabolism. Opsin 3 was shown to regulate thermogenesis and lipolysis via a blue-light dependent mechanism in adipose tissue. 5
As mentioned above, Opsin 3 is extensively expressed in several brain regions, including cortex, hippocampus and hypothalamus. Opsin 3 expression in brain regions associated with circadian rhythms, such as the hypothalamus, may suggest a possible role in regulating these rhythms. This connection could influence sleep-wake cycles and other circadian-related functions. In addition, Opsin 3 expression in regions like the hippocampus, a key area for memory and learning, indicates it might play a role in neural development or synaptic plasticity. This could have implications for learning, memory, and other cognitive functions. 4,6
Finally, Opsin 3 has been detected in several cells of the immune system including T-cells and macrophages, implying that immune responses could be potentially regulated through light-related mechanisms.2,7
Anti-Opsin 3 (extracellular) Antibody (#AOR-023) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Opsin 3 from mouse, rat and human samples.
Powered by Bioz
