Overview
- Peptide (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R (Accession P56718). 3rd intracellular loop.
Multiplex staining of OX1R and Parathyroid hormone 1 receptor in rat ventromedial hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#AOR-001-AG), (1:60) and Anti-PTH1R (extracellular) Antibody (#APR-051), (1:60). A. Sections were incubated with Anti-PTH1R (extracellular) Antibody, followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody, (green). C. Merge of A and B shows staining of OX1R in nerve fibers (vertical arrows) while Parathyroid hormone 1 receptor is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- Sakurai, T. et al. (1998) Cell 92, 573.
- Kukkonen, J.P. et al. (2002) Am. J. Physiol. Cell. Physiol. 283, C1567.
- Kirchgessner, A.L. (2002) Endocr. Rev. 23, 1.
- Sakurai, T. (2007) Nat. Rev. Neurosci. 8, 171.
Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.
OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3
Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3
The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.
OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3
The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4
Anti-Orexin Receptor 1 Antibody (#AOR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples.
Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#AOR-001-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.
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