Name: Anti-OXGR1/GPR99 Antibody
Brand: Alomone Labs
SKU: AOR-016

Overview

Cat #: AOR-016

Alternative Name Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide CKASGDLEQGKKDS, corresponding to amino acid residues 319-332 of mouse OXGR1 (Accession Q6IYF8). Intracellular, C-terminus.

Accession (Uniprot) Number Q6IYF8

Gene ID 239283

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – 12/14 amino acid residues identical; human – 9/14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse kidney (lanes 1 and 4), rat kidney (lanes 2 and 5) (1:600) and rat testis (lanes 3 and 6) (1:200) lysates:
1-3. Anti-OXGR1/GPR99 Antibody (#AOR-016).
4-6. Anti-OXGR1/GPR99 Antibody, preincubated with OXGR1/GPR99 Blocking Peptide (#BLP-OR016).

Immunohistochemistry

Expression of OXGR1/GPR99 in mouse hippocampus and cerebellum
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-OXGR1/GPR99 Antibody (#AOR-016), (1: 200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR99 staining (green) in hippocampal dentate gyrus, is detected in the granule layer (G, vertical arrows) and in neurons of the hilus (H, horizontal arrows). B. GPR99 immunoreactivity (green) in cerebellum, is observed in neurons of the purkinje cell layer (P, vertical arrows). Cell nuclei are stained with DAPI (blue).

References

Lazo-Fernandez, Y. et al. (2008) Am. J. Physiol. 315, F7.
Golay, H. et al. (2019) Int. J. Mol. Sci. 20, 3889.
Tokonami, N. et al. (2013) J. Clin. Invest. 123, 3166.
He, W. et al. (2004) Nature 429, 188.
Omede, A. et al. (2016) Biochem. Biophys. Res. Commun. 479, 708.

Scientific background

Oxoglutarate receptor 1 (OXGR1) is a receptor for α-ketoglutarate (αKG), a metabolite that is a citric acid cycle intermediate and a glutamine catabolism by-product¹. αKG also serves as a metabolic marker and its interaction with OXGR1 has been shown to mediate the adaptive regulation of HCO₃^– secretion and NaCl reabsorption under acid-base stress³.

OXGR1 belongs to the G-protein coupled receptor (GPCRs) protein family. Members of this group contain seven trans-membrane domains and respond to a large variety of ligands and thereby affect key cellular functions that are crucial for cell survival or proliferation². OXGR1 belongs to a subset of metabolic GPCRs, along with receptors for succinate (GPR91), lactate (GPR81), fatty acids (FFAR) and many more³.

OXGR1 is expressed in the kidney^3,4 and in the heart, where it affects the contractile function, consistent with the finding that α-ketoglutarate levels are high in heart failure patients⁵.

Because of its major role in maintaining cellular balance and its role in heart contractile activity, identification of molecules that can specifically activate or inhibit this receptor may be very useful in the development of novel therapeutic approach for pathological cardiac hypertrophy⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-OXGR1/GPR99 Antibody (#AOR-016) is a highly specific antibody directed against an epitope of the mouse Oxoglutarate receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize OXGR1 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

OXGR1/GPR99 Blocking Peptide (#BLP-OR016) is the original antigen used for immunization during Anti-OXGR1/GPR99 Antibody (#AOR-016) generation. The blocking peptide binds and ‘blocks’ Anti-OXGR1/GPR99 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

OXGR1/GPR99 Blocking Peptide (#BLP-OR016)

Shipping and Ordering Information

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Anti-OXGR1/GPR99 Antibody