Overview
- Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor (Accession P49653). Intracellular, C-terminus.
- Rat brain membranes, PC12 cells lysate (1:200).
Western blot analysis of differentiated (lanes 1 and 3) and non-differentiated (lanes 2 and 4) PC12 cell lysates:1,2. Anti-P2X2 Receptor Antibody (#APR-003), (1:200).
3,4. Anti-P2X2 Receptor Antibody, preincubated with P2X2 Receptor Blocking Peptide (#BLP-PR003).
- Rat brain membranes (2 μg) (Masin, M. et al. (2006) J. Biol. Chem. 281, 4100.).
- Rat neocortex frozen section. Mouse ilieum (1:200) (Ren, J. et al. (2003) J. Physiol. 552, 809.).
- Cultured rat myeteric cells (1:200) (Ohta, T. et al. (2005) Am. J. Physiol. 289, G935.).
- Human CD4+ and ckit+ cells (Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.).
- The control antigen is not suitable for this application.
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.
The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.
Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9
The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.
mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10
Expression of P2X2 receptor in rat SCN.Immunocytochemical staining of rat suprachiasmatic nuclei (SCN) cell culture. Fixed and permeabilized cells were stained using Anti-P2X2 Receptor Antibody (#APR-003). P2RX2 immunoreactivity (red) is observed in punctate structures in the SCN cell culture. Arrowheads indicate example structures that are double-labeled with P2RX2 and synapsin I (green). Dapi is used to label cell nuclei. Scale bar, 20 μm.Adapted from Bhattacharya, A. et al. (2013) with permission of The Society for Neuroscience.
