Name: Anti-P2X3 Receptor-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: APR-016-AR

Overview

Cat #: APR-016-AR

Alternative Name P2RX3, P2X purinoceptor 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor (Accession P49654). Intracellular, C-terminus.

Accession (Uniprot) Number P49654

Gene ID 81739

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 13/15 amino acid residues identical.

RRID AB_2341047.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-016), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Expression of P2RX3 in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-P2X3 Receptor-ATTO Fluor-594 Antibody (#APR-016-AR). A. P2X3 staining (red) is detected in DRG neurons. B. Nuclear staining using DAPI as the counterstain (Blue). C. Merge images of A and B.

References

Florenzano, F. et al. (2002) Neuroscience 115, 425.
Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed.1.
Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
Kage, K. et al. (2002) Exp. Brain Res. 147, 511.
Prasad, M. et al. (2001) J. Physiol. 573, 667.
Aoki, Y. et al. (2003) Brain Res. 989, 214.
Burnstock, G. (2000) Br. J. Anaesth. 84, 476.

Scientific background

The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.^1,2,3

All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.^4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.^6,7Involvement of ATP in the mechanism of chronic pain has been also suggested.^7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2X3 Receptor Antibody (#APR-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.

Anti-P2X3 Receptor-ATTO Fluor-594 Antibody (#APR-016-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-P2X3 Receptor-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 13/02/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

P2X3 Receptor Blocking Peptide (#BLP-PR016) is the original antigen used for immunization during Anti-P2X3 Receptor Antibody (#APR-016) generation. The blocking peptide binds and ‘blocks’ Anti-P2X3 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

P2X3 Receptor Blocking Peptide (#BLP-PR016)

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Anti-P2X3 Receptor-ATTO Fluor-594 Antibody