Name: Anti-P2X3 Receptor (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: APR-026-F

Overview

Cat #: APR-026-F

Alternative Name P2RX3, P2X purinoceptor 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.

Accession (Uniprot) Number P49654

Gene ID 81739

Homology Mouse – identical; human - 13/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-026), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of P2X3 Receptor by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-P2X3 Receptor (extracellular)-FITC Antibody (#APR-026-F), (5µg).
Cell surface detection of P2X3 Receptor by direct flow cytometry in live intact mouse P815 mastocytoma cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-P2X3 Receptor (extracellular)-FITC Antibody (#APR-026-F), (5µg).
Cell surface detection of P2X3 Receptor by direct flow cytometry in live intact human KU812 basophil cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.(#RIC-001-F)
^___ Cells + Anti-P2X3 Receptor (extracellular)-FITC Antibody (#APR-026-F), (5µg).

References

Florenzano, F. et al. (2002) Neuroscience 115, 425.
Ashcroft, F.M. et al. (1999) Ion Channels and Disease Ed.1.
Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
Kage, K. et al. (2002) Exp. Brain Res. 147, 511.
Prasad, M. et al. (2001) J. Physiol. 573, 667.
Aoki, Y. et al. (2003) Brain Res. 989, 214.
Burnstock, G. (2000) Br. J. Anaesth. 84, 476.

Scientific background

The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.^1,2,3

All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.^4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.^6,7Involvement of ATP in the mechanism of chronic pain has been also suggested.^7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2X3 Receptor (extracellular) Antibody (#APR-026) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize P2X3 receptor from rat, mouse and human samples.

Anti-P2X3 Receptor (extracellular)-FITC Antibody (#APR-026-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize P2X3 receptor from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

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Anti-P2X3 Receptor (extracellular)-FITC Antibody