Overview
- Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
Western blot analysis of rat DRG:1. Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (1:200).
2. Anti-P2X3 Receptor (extracellular) Antibody, preincubated with P2X3 Receptor (extracellular) Blocking Peptide (#BLP-PR026).
Expression of P2RX3 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), followed by anti-rabbit-AlexaFluor-594 secondary antibody. P2RX3 staining (red) appears in neuronal cell bodies. DAPI was used as the counter stain (blue). A. X20 magnification. B. X40 magnification.
Cell surface detection of P2X3 Receptor by indirect flow cytometry in live intact mouse P815 mastocytoma cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (2.5μg) + goat-anti-rabbit-FITC.
Expression of P2RX3 in rat PC12 cellsCell surface detection of P2RX3 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining with Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (1:50, red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI was used as the counter stain (blue). C. Merge images of A and B.
- Florenzano, F. et al. (2002) Neuroscience 115, 425.
- Ashcroft, F.M. et al. (1999) Ion Channels and Disease Ed.1.
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- Aoki, Y. et al. (2003) Brain Res. 989, 214.
- Burnstock, G. (2000) Br. J. Anaesth. 84, 476.
The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3
All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.
Anti-P2X3 Receptor (extracellular) Antibody (#APR-026) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.
Applications
Citations
Powered by Bioz- Western blot and immunohistochemistry of rat stellate ganglia. Tested in P2X3 shRNA knockdown rats.
Sheng, X. et al. (2018) Neurochem. Int. 120, 206.
- Rat DRG lysate (1:2000).
Gu, Y. et al. (2016) Mol. Pain 12, 1.
- Rat DRGs (1:100).
Gu, Y. et al. (2016) Mol. Pain 12, 1.
