Name: Anti-P2X3 Receptor Antibody
Brand: Alomone Labs
SKU: APR-016

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Overview

Cat #: APR-016

Alternative Name P2RX3, P2X purinoceptor 3, ATP Receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor (Accession P49654). Intracellular, C-terminus.

Accession (Uniprot) Number P49654

Gene ID 81739

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 13/15 amino acid residues identical.

RRID AB_2313760.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, ih, wb

May also work in: ip*

Western blot

Rat DRG lysates (1:200-1:500).
Western blot analysis of rat DRG lysates:
1. Anti-P2X3 Receptor Antibody (APR-016), (1:200).
2. Anti-P2X3 Receptor Antibody, preincubated with P2X3 Receptor Blocking Peptide (#BLP-PR016).

Immunohistochemistry

Rat embryo DRG formalin frozen sections.

Immunocytochemistry

Fixed mouse trigeminal neurons (1:200) (D'Arco, M. et al. (2007) J. Neurosci. 27, 8190.).

Indirect flow cytometry

Human CD4+ and ckit+ cells (Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.).

Scientific background

The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.^1,2,3

All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.^4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.^6,7Involvement of ATP in the mechanism of chronic pain has been also suggested.^7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

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Anti-P2X3 Receptor Antibody