Name: Anti-P2X5 Receptor (extracellular) Antibody
Brand: Alomone Labs
SKU: APR-027

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Overview

Cat #: APR-027

Alternative Name P2RX5, P2X purinoceptor 5

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)HRSHTRLAEMKYE, corresponding to amino acid residues 299-311 of rat P2X5 receptor (Accession P51578).

Accession (Uniprot) Number P51578

Gene ID 303211

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse –12/13 amino acid residues; human – 10/13 amino acid residues.

RRID AB_2756757.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Rat and mouse heart membranes (1:400-1:2000).
Western blot analysis of rat heart (lanes 1 and 3) and mouse heart (lanes 2 and 4):
1,2. Anti-P2X5 Receptor (extracellular) Antibody (#APR-027), (1:400).
3,4. Anti-P2X5 Receptor (extracellular) Antibody, preincubated with P2X5 Receptor (extracellular) Blocking Peptide (#BLP-PR027).

Indirect flow cytometry

Cell surface detection of P2X5 Receptor by indirect flow cytometry in live intact mouse WEHI B-cell lymphoma cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-P2X5 Receptor (extracellular) Antibody (#APR-027), (2.5μg) + goat-anti-rabbit-FITC.

Scientific background

The P2X5 receptor (P2X5R) belongs to the ligand-gated ion channel P2X family, which is activated by extracellular ATP. To date, seven receptor subtypes named P2X1-P2X7 have been described.^1,2,3

All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.^4-5 The P2X5R forms a heteromeric receptor with P2X1 receptor. The heteromeric receptor has distinct properties from the P2X1 or P2X5 homomeric receptors.^4,6 The different P2X receptors show distinct expression patterns.

P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and in microglia.

The P2X5 subunit was first cloned from rat heart, and was reported to be expressed in heart, brain, spinal cord and adrenal gland.

Recent papers suggested possible roles for P2X5 receptor in differentiation and apoptosis in keratinocytes and ageing rat prostate, respectively.^7-10

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

P2X5 Receptor (extracellular) Blocking Peptide (#BLP-PR027) is the original antigen used for immunization during Anti-P2X5 Receptor (extracellular) Antibody (#APR-027) generation. The blocking peptide binds and ‘blocks’ Anti-P2X5 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

P2X5 Receptor (extracellular) Blocking Peptide (#BLP-PR027)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Certificate of Analysis

Anti-P2X5 Receptor (extracellular) Antibody