Overview
Alternative Name P2RX6, P2X purinoceptor 6, P2XM, Purinergic receptor P2X-like 1, P2RXL1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)RTKYEEARAPKATTNSA, corresponding to amino acid residues 363-379 of rat P2X6 receptor (Accession P51579). Intracellular, C-terminus.
Accession (Uniprot) Number P51579
Gene ID 25041
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 15/17 amino acid residues identical; human - 14/17 amino acid residues identical.
RRID AB_2040062.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.7 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, wb
May also work in: ip*
Western blot
Western blot analysis of rat brain membranes:1. Anti-P2X6 Receptor Antibody (#APR-013), (1:200).
2. Anti-P2X6 Receptor Antibody, preincubated with P2X6 Receptor Blocking Peptide (#BLP-PR013).
Immunohistochemistry
- Rat lens (1:200) (Suzuki Kerr, H. et al. (2009) Histochem. Cell. Biol. 131, 667.).
Expression of P2X6 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-P2X6 Receptor Antibody (#APR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. P2X6 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with P2X6 Blocking Peptide (BLP-PR013), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle.
Expression of P2X6 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-P2X6 Receptor Antibody (#APR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. P2X6 immunoreactivity (green) appears in cortical neurons (arrows). B. Pre-incubation of the antibody with P2X6 Blocking Peptide (BLP-PR013), suppressed staining. Cell nuclei are stained with DAPI (blue).
Immunocytochemistry
- Mouse Leydig cells (1:200) (Subitoni, L. et al. (2009) Purinergic Signal. 5, 277.).
Indirect flow cytometry
- Human CD4+ and ckit+ cells (Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.).
References
- Prasad, M. et al. (2001) J. Physiol. 537, 667.
- Florenzano, F. et al. (2002) Neuroscience 115, 425.
- Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
- Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
- Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
- Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
- Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
- Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
- Kim, M. et al. (2001) EMBO J. 20, 6347.
Scientific background
P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP. P2X receptors are involved in synaptic communication and in diseases of the nervous system. The P2X receptor family consists of at least seven isoforms: P2X1-P2X7.1,2,3
All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9
The P2X6 receptor assembles to a heteromultimer with two other P2X subunits, P2X2 and P2X4.
P2RX6 is extensively expressed throughout the central nervous system.7
Lyophilized Powder
Anti-P2X6 Receptor Antibody (#APR-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize P2RX6 from human, mouse, and rat samples.
For research purposes only, not for human use
Last Update: 29/10/2024
Applications
Citations
Citations
Powered by BiozMore product citations
- Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.
- Subitoni, L. et al. (2009) Purinergic Signal. 5, 277.
- Suzuki Kerr, H. et al. (2009) Histochem. Cell. Biol. 131, 667.
