Name: Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody
Brand: Alomone Labs
SKU: APR-008-AG

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Overview

Cat #: APR-008-AG

Alternative Name Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.

Accession (Uniprot) Number Q9Z1M0

Gene ID 18439

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, rat - identical; bovine - 14/17 amino acid residues identical.

RRID AB_2341045.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-008), and direct flow cytometry (labeled antibody).

Applications: fc, ic, if, ih, lci

Immunohistochemistry

Mouse muscle-myenteric plexus sections (1:100) (Caputi, V. et al. (2017) Front. Pharmacol. 8, 350.).

Live cell imaging / Immunocytochemistry

Transfected HEK-293 cells (1:25).
Expression of P2RX7 in HEK-293 transfected cells
Cell surface detection of P2RX7 in live intact P2X7-HEK-293 transfected cells. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG), (green), (1:25).

Scientific background

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.

The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.^1-3

Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.⁴

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Expression of P2RX7 in rat colon.Immunohistochemical staining of rat distal colon section using Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG). A. P2X7 staining (green) in normal colon sections co-localizes with that of HuC/D (red). B. P2X7 staining in the presence of colitis is detected in the neuromuscular layer and expression of the receptor is increased in myenteric ganglia. TOTO-3 (blue) was used to stain nuclei.Adapted from Antonioli, L. et al. (2014) PLoS One 9, e116253. with kind permission of Dr. Colucci, R. Div. Pharmacology and Chemotherapy, Dept. of Clinical and Experimental Medicine, University of Pisa Pisa, Italy.

For research purposes only, not for human use

Last Update: 03/01/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Products

P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008) is the original antigen used for immunization during Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) generation. The blocking peptide binds and ‘blocks’ Anti-P2X7 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008)
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG)

Shipping and Ordering Information

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Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody