Overview
Alternative Name Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
Accession (Uniprot) Number Q64663
Gene ID 29665
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.
RRID AB_2040067.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-004), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
- Rat pancreas paraffin embedded section (1:20). Rat spinal cord (1:100). Mouse spinal cord sections (1:20) (Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102.).
Multiplex staining of VGLUT2 and P2X7 Receptor in rat spinal cordImmunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-VGLUT2 Antibody (#AGC-036), (1:600) and Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO), (1:100). A. Vesicular Glutamate Transporter 2 labeling followed by goat-anti-rabbit-Alexa-488 (green). B. The same section labeled for P2X7 Receptor (orange). C. Merge of A and B demonstrates partial co-localization of VGLUT2 and P2X7 Receptor in dorsal horn and in lateral column (L. Col., arrow). Cell nuclei were stained with DAPI (blue).
Scientific background
The P2X7 purinergic receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family consists of seven receptor subtypes, named P2X1-P2X7, all of which have been cloned.
The various P2X receptors show distinct expression patterns. P2X1-6 receptors have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly in antigen-presenting cells, and microglia.
The P2X7 receptor mediates the release of proinflammatory cytokines and stimulation of transcription factors and may also play an important role in apoptosis.1-3
Lyophilized Powder
Image & Title: 
Expression of P2X7 purinergic receptor in mouse spinal cordImmunohistochemical staining of mouse spinal cord sections (L3–L5) from SOD1-G93A mice were double-immunostained either with anti-Iba-1, NeuN or GFAP (green) and Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO), (red). P2X7 is present only in microglia cells as shown in the merged panel (yellow). Scale bar = 20 μm, insets = 50 μm.Adapted from Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102. with permission of Oxford University Press.
Expression of P2X7 purinergic receptor in mouse spinal cordImmunohistochemical staining of mouse spinal cord sections (L3–L5) from SOD1-G93A mice were double-immunostained either with anti-Iba-1, NeuN or GFAP (green) and Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO), (red). P2X7 is present only in microglia cells as shown in the merged panel (yellow). Scale bar = 20 μm, insets = 50 μm.Adapted from Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102. with permission of Oxford University Press.
For research purposes only, not for human use
Last Update: 13/02/2024
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