Anti-P2X7 Receptor (extracellular)-mFluor™ Violet 450 Antibody

Overview

Cat #: APR-008-V

Alternative Name Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: H, M, R

Immunogen

Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.

Accession (Uniprot) Number Q9Z1M0

Gene ID 18439

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, rat - identical; bovine - 14/17 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label mFluor™ Violet 450. Maximum absorption 405 nm and maximum emission 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-008), and direct flow cytometry (labeled antibody).

Applications: FC, LCI

Direct flow cytometry

Cell surface detection of P2X7 Receptor by direct flow cytometry in live intact mouse BV2 microglia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-mFluor™ Violet 450 (#RIC-001-V).
^___ Cells + Anti-P2X7 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-008-V), (1µg).
Cell surface detection of P2X7 Receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-mFluor™ Violet 450 (#RIC-001-V).
^___ Cells + Anti-P2X7 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-008-V), (2.5µg).

References

Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
Kim, M. et al. (2001) EMBO J. 20, 6347.
Chizh, B.A. and Illes, P. (2001) Pharmacol. Rev. 53, 553.
Nihei, O.K. et al. (2000) Mem.Inst.Oswaldo Cruz. 95, 415.

Scientific background

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.

The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.^1-3

Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.⁴

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.

Anti-P2X7 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-008-V) is directly conjugated to mFluor™ Violet 450 fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

mFluor™ Violet 450 dye has a maximum absorption of 405 nm and maximum emission of 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 19/02/2025

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-mFluor™ Violet 450 (#RIC-001-V), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to mFluor™ Violet 450.

Rabbit IgG Isotype Control-mFluor™ Violet 450 (#RIC-001-V)

Shipping and Ordering Information

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