Name: Anti-P2X7 Receptor (extracellular)-PE Antibody
Brand: Alomone Labs
SKU: APR-008-PE

Overview

Cat #: APR-008-PE

Alternative Name Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.

Accession (Uniprot) Number Q9Z1M0

Gene ID 18439

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, rat - identical; bovine - 14/17 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Label R-Phycoerythrin (R-PE).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-008), and direct flow cytometry (labeled antibody).

Applications: fc

May also work in: ic*, lci*

Direct flow cytometry

Cell surface detection of P2RX7 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-PE.
^___ Cells + Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE), (2.5 µg).
Cell surface detection of P2RX7 in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-PE.
^___ Cells + Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE), (5 µg).

References

Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
Kim, M. et al. (2001) EMBO J. 20, 6347.
Chizh, B.A. et al. (2000) Pharmacol. Rev. 53, 553.
Nihei, O.K. et al. (2000) Mem. Inst. Oswaldo Cruz. 95, 415.

Scientific background

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.

The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis^1-3.

Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization, and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx, and cell phenotype⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.

Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-PE (#RIC-001-PE), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Phycoerythrin (PE) fluorophore.

Rabbit IgG Isotype Control-PE (#RIC-001-PE)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-P2X7 Receptor (extracellular)-PE Antibody