Overview
- Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Western blot analysis of rat brain membranes (lanes 1 and 5) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 6) and Mouse WEHI-231 B cell lymphoma (lanes 3 and 7) and human HL-60 promyelocytic leukemia cell line (lanes 4 and 8):1-4. Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:200).
5-8. Anti-P2X7 Receptor (extracellular) Antibody, preincubated with P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008).
Expression of P2RX7 in rat pancreasImmunohistochemical staining of rat pancreas frozen section using Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:100), followed by mouse anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.
- Mouse P2X7 transfected in CHO cells (1:500). (Adriouch, S. et al. (2005) Cell. Immunol. 236, 72.).
Cell surface detection of P2X7 Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (2.5μg) + goat-anti-rabbit-FITC.- The control antigen is not suitable for this application.
Expression of P2RX7 in rat RBL cellsCell surface detection of P2RX7 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
- Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
- Kim, M. et al. (2001) EMBO J. 20, 6347.
- Chizh, B.A. and Illes, P. (2001) Pharmacol. Rev. 53, 553.
- Nihei, O.K. et al. (2000) Mem.Inst.Oswaldo Cruz. 95, 415.
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.
The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3
Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4
Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.
Expression and upregulation of microglial P2X7 receptor in cancer.A. Immunohistochemical staining of of rat spinal dorsal horn using Anti-P2X7 Antibody (#APR-004) or Anti-P2X7 (extracellular) Antibody (#APR-008) shows that P2X7 co-localizes with CD11b, a microglia marker (two panels) and not with NeuN, a neuronal marker (lower right panel). Specificity of the antibody was tested on P2X7 knock-out mice (western blot, in upper panel). B. Western blot analysis of rat dorsal horn lysates shows that P2X7 expression increases on post-tumor day 14 (PTD 14).Adapted from Yang, Y. et al. (2015) with permission of the Society for Neuroscience.
Applications
Citations
Powered by Bioz- Western blot analysis of mouse neutrophil lysate. Tested in P2X7-/- mice.
Karmakar, M. et al. (2016) Nat. Commun. 7, 10555.
- HEK 293 transfected cells (1:10000).
Migita, K. et al. (2016) Mol. Pharmacol. 90, 116. - Mouse neutrophil lysate. Also tested on P2X7-/- mice.
Karmakar, M. et al. (2016) Nat. Commun. 7, 10555. - Mouse MC3T3-E1 cells lysate (preosteoblast-like cell line).
Gangadharan, V. et al. (2015) Am. J. Physiol. 308, C41. - Rat eye lysate (1:500).
Sugiyama, T. et al. (2013) Mol. Vis 19, 2080. - Human immortalized gingival keratinocyte (HIGK) cell lysate.
Hung, S.C. et al. (2013) PLoS ONE 8, e70210. - Equine hoof lysate (1:200).
Zamboulis, D.E. et al. (2013) Purinergic Signal. 9, 383. - Human mesenchymal stem cells (hMSCs).
Sun, D. et al. (2013) Stem Cells 31, 1170.
- Mouse T-cells.
Elkhal, A. et al. (2016) Sci. Rep. 6, 1.
- Mouse retinal sections.
Hu, S.J. et al. (2015) J. Neurosci. 35, 6987. - Rat retina sections (1:200).
Sugiyama, T. et al. (2013) Mol. Vis 19, 2080. - Equine palmar digital artery and Schwann cells (1:200).
Zamboulis, D.E. et al. (2013) Purinergic Signal. 9, 383. - Primate retina (1:500).
Gu, B.J. et al. (2013) FASEB J. 27, 1479.
- HEK 293 transfected cells (1:1000).
Migita, K. et al. (2016) Mol. Pharmacol. 90, 116. - Human mesenchymal stem cells (hMSCs) (1:100).
Sun, D. et al. (2013) Stem Cells 31, 1170. - Mouse P2X7 transfected in CHO cells (1:500).
Adriouch, S. et al. (2005) Cell. Immunol. 236, 72.
- Antibody was used to block P2X7-dependent PGE2 release.
Li, J. et al. (2005) J. Biol. Chem. 280, 42952.
- Takai, E. et al. (2012) J. Cell Sci. 125, 5051.
