Overview
- Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
- Rat hippocampus lysate (1:200-1:1000).
Western blot analysis of rat hippocampus lysates:1. Anti-P2X7 Receptor Antibody (#APR-004), (1:200).
2. Anti-P2X7 Receptor Antibody, preincubated with P2X7 Receptor Blocking Peptide (#BLP-PR004).
- Mouse brain, lung and submandibular glands (Sim, J.A. et al. (2004) J. Neurosci. 24, 6307.).
- Rat brain sections (see also 3-5 in recent publications using this product).
- Mouse pancreatic stellate cells (PSCs), (1:100), (Haanes, K.A. et al. (2012) PLoS ONE 7, e51164.).
Human MG63 and SaOS2 osteosarcoma cell lines (Alqallaf, S.M. et al. (2009) Br. J. Pharmacol. 156, 1124.).
The P2X7 purinergic receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family consists of seven receptor subtypes, named P2X1-P2X7, all of which have been cloned.
The various P2X receptors show distinct expression patterns. P2X1-6 receptors have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly in antigen-presenting cells and microglia.
The P2X7 receptor mediates the release of proinflamatory cytokines and stimulation of transcription factors and may also play an important role in apoptosis.1-3
Knockout validation of Anti-P2X7 Receptor Antibody in mouse spinal cord.Western blot analysis of mouse total lumbar spinal cord lysates (L3–L5) from WT, P2X7+/- and P2X7-/- 90-day-old mice using Anti-P2X7 Receptor Antibody (#APR-004).Adapted from Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102. with permission of Oxford University Press.
