Overview
Alternative Name P2Y purinoceptor 1, P2RY1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900). 2nd extracellular loop.
Accession (Uniprot) Number P47900
Gene ID 5028
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; mouse - 12/14 amino acid residues identical.
RRID AB_10918468.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-021), and Direct Flow Cytometry (labeled antibody).
Applications: fc, ic, if, ih, lci
Immunohistochemistry
- Mouse brain sections (1:80).
Direct flow cytometry
- Jurkat living cells 5 µg/5x105 cells.
Live cell imaging / Immunocytochemistry
- Rat PC12 cells.
Expression of P2RY1 in rat PC12 cellsCell surface detection of P2RY1 in intact living rat pheochromocytoma (PC12) cells. Extracellular staining of cells using Anti-P2Y1 receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-021-AG), (1:50), (green).
Scientific background
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4
P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5
Lyophilized Powder
For research purposes only, not for human use
Last Update: 03/01/2024
Specifications
Citations
Citations
