Overview
Alternative Name P2Y purinoceptor 1, P2RY1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: H, M, R
Immunogen
- Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900). 2nd extracellular loop.
Accession (Uniprot) Number P47900
Gene ID 5028
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; mouse - 12/14 amino acid residues identical.
RRID AB_10919250.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ICC, IF, IFC, IHC, LCI, WB
Cited Applications: IFC*
Western blot
- Rat brain, K562 (human chronic myelogeneous leukemia) and Jurkat (human acute T cell leukemia) lysates (1:200-1:400).
Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates:1-3. Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:200).
4-6. Anti-P2Y1 Receptor (extracellular) Antibody, preincubated with P2Y1 Receptor (extracellular) Blocking Peptide (#BLP-PR021).
Immunohistochemistry
- Mouse brain sections.
Indirect flow cytometry
- Jurkat intact living cells (5 μg/1x106 cells).
- The control antigen is not suitable for this application.
Live cell imaging / Immunocytochemistry
- Rat PC12 cells (1:50).
Scientific background
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4
P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5
Lyophilized Powder
For research purposes only, not for human use
Last Update: 25/03/2025
Specifications
Citations
Citations
