Overview
- Peptide (C)NATAAPKPSEPQSRELS, corresponding to amino acid residues 357-373 of human P2RY11 (Accession Q96G91). Intracellular, C-terminus.
Western blot analysis of human platelet lysates:1. Anti-Human P2Y11 Receptor Antibody (#APR-015), (1:200).
2. Anti-Human P2Y11 Receptor Antibody, preincubated with Human P2Y11 Receptor Blocking Peptide (#BLP-PR015).
- Mouse bone marrow lysate (Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.).
Expression of P2Y11 receptor in human P2Y11-MDCK transfected cellsImmunocytochemical staining of paraformaldehyde-fixed and permeabilized human P2Y11-MDCK transfected cells. A. Staining using Anti-Human P2Y11 Receptor Antibody (#APR-015), (1:200), followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.
- Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The blocking peptide is not suitable for this application.
- Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
- Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
- Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52.
- Communi, D. et al. (2000) FEBS Letters 475, 39.
- White, P.J. et al. (2003) Mol. Pharmacol. 63, 1356.
- Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3
The human P2Y11 receptor is unique among the P2Y receptors since it is dually coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly expressed in the spleen and to a much lower extent in platelets.
Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4
Anti-Human P2Y11 Receptor Antibody (#APR-015) is a highly specific antibody directed against an epitope of the human P2RY11. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunocytochemistry applications. It has been designed to recognize P2RY11 from human samples.
Applications
Citations
Powered by Bioz- Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat cardiac fibroblast lysate (1:200).
Certal, M. et al. (2015) Cell Calcium 58, 518. - Human PBMC lysate (1:600).
Chadet, S. et al. (2015) J. Immunol. 195, 651. - Human derived keratinocytes (HaCaT cells), (1:200).
Ishimaru, M. et al. (2013) Eur. J. Pharmacol. 703, 67.
- Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
- Rat cardiac fibroblasts (1:50).
Certal, M. et al. (2015) Cell Calcium 58, 518.
- Mouse bone marrow cells.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat osteoclasts (6 μg/ml).
Alvarenga, E.C. et al. (2010) Bone 46, 355.
- Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
