Overview
Alternative Name P2Y purinoceptor 11, P2RY11
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
- Peptide (C)NATAAPKPSEPQSRELS, corresponding to amino acid residues 357-373 of human P2RY11 (Accession Q96G91). Intracellular, C-terminus.
Accession (Uniprot) Number Q96G91
Gene ID 5032
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only. Not recommended for use with rat and mouse samples.
RRID AB_2040072.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.65 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ip, wb
May also work in: ih*
Western blot
- Human platelet lysates (1:200)
Western blot analysis of human platelet lysates:1. Anti-Human P2Y11 Receptor Antibody (#APR-015), (1:200).
2. Anti-Human P2Y11 Receptor Antibody, preincubated with Human P2Y11 Receptor Blocking Peptide (#BLP-PR015).
Immunoprecipitation
- Mouse bone marrow lysate (Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.).
Immunocytochemistry
- Human P2Y11-MDCK transfected cells (1:200).
Indirect flow cytometry
- Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The blocking peptide is not suitable for this application.
Scientific background
The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3
The human P2Y11 receptor is unique among the P2Y receptors since it is dually coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly expressed in the spleen and to a much lower extent in platelets.
Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
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