Name: Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: APR-012-AR

Overview

Cat #: APR-012-AR

Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12 (Accession Q9H244). Intracellular, 2^nd cytoplasmic loop.

Accession (Uniprot) Number Q9H244

Gene ID 64805

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - 16/18 amino acid residues identical.

RRID AB_10918469.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-012), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Expression of P2RY12 in rat striatum
Immunohistochemical staining of rat striatum using Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR). P2Y12 (red) appears in patches (white arrows) of myelinated axons. DAPI is used as the counterstain (blue).

References

Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52.
Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.

Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1,P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.^1-3

The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca²⁺ upon activation.

The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.⁴ The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.⁴

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2Y12 Receptor Antibody (#APR-012) is a highly specific antibody directed against an epitope of the human P2RY12. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize P2RY12 from human, rat, and mouse samples.

Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 13/02/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

P2Y12 Receptor Blocking Peptide (#BLP-PR012) is the original antigen used for immunization during Anti-P2Y12 Receptor Antibody (#APR-012) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y12 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

P2Y12 Receptor Blocking Peptide (#BLP-PR012)

Shipping and Ordering Information

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Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody