Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-012), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
Rat striatum frozen section (1:25).
Expression of P2RY12 in rat striatum
Immunohistochemical staining of rat striatum using Anti-P2Y12 Receptor-ATTO Fluor-594Antibody (#APR-012-AR). P2Y12 (red) appears in patches (white arrows) of myelinated axons. DAPI is used as the counterstain (blue).
Scientific background
The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1,P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3
The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.
The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Expression of P2RY12 in rat striatumImmunohistochemical staining of rat striatum using Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR). P2Y12 (red) appears in patches (white arrows) of myelinated axons. DAPI is used as the counterstain (blue).