Overview
- Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2Y12 receptor (Accession Q9H244). 3rd extracellular loop.
- Cell surface detection of P2Y12 by direct flow cytometry in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-APC (#RIC-001-APC).
___ Cells + Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC), (2.5µg). - Cell surface detection of P2Y12 by direct flow cytometry in live intact human MEG-01 megakaryocytic cells:___ Cells.
___ Cells + Rabbit IgG isotype control-APC (#RIC-001-APC).
___ Cells + Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC), (2.5µg). - Cell surface multicolor flow cytometry analysis of live intact mouse BV-2 microglia cells with P2Y12 and TREM2:BV-2 cells were labeled with Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC), (5µg) and with Anti-TREM2 (extracellular)-PE Antibody (#ANR-018-PE), (5µg).
- Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
- Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
- Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
- Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.
The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3.
The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.
The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.
Application key:
Species reactivity key:
Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize P2Y12 from human, mouse, and rat samples.
Anti-P2Y12 Receptor (extracellular)-APC Antibody (#APR-020-APC) is directly conjugated to the Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.