Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody

Overview

Cat #: APR-020-V

Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: H, M, R

Immunogen

Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3^rdextracellular loop.

Accession (Uniprot) Number Q9H244

Gene ID 64805

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - 12/13 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label mFluor™ Violet 450. Maximum absorption 405 nm and maximum emission 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-020), and direct flow cytometry (labeled antibody).

Applications: FC, LCI

Direct flow cytometry

Cell surface detection of P2RY12 by direct flow cytometry in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-mFluor™ Violet 450 (#RIC-001-V).
^___ Cells + Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-020-V), (2.5µg).
Cell surface detection of P2RY12 by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-mFluor™ Violet 450 (#RIC-001-V).
^___ Cells + Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-020-V), (2.5µg).
Cell surface detection of P2RY12 by direct flow cytometry in live intact mouse SIM-A9 microglia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-mFluor™ Violet 450 (#RIC-001-V).
^___ Cells + Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-020-V), (2.5µg).

References

Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.

Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14^1-3.

The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca²⁺ upon activation.

The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting⁴. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human P2RY12. The antibody can be used in western blot, live cell imaging and indirect flow cytometry applications. It has been designed to recognize P2RY12 from human, rat and mouse samples.

Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-020-V) is directly conjugated to mFluor™ Violet 450 fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

mFluor™ Violet 450 dye has a maximum absorption of 405 nm and maximum emission of 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 19/02/2025

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-mFluor™ Violet 450 (#RIC-001-V), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to mFluor™ Violet 450.

Rabbit IgG Isotype Control-mFluor™ Violet 450 (#RIC-001-V)

Shipping and Ordering Information

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