Overview
- Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.
- Rat brain and MEG-01 cell line (human chronic myelogenous leukemia) lysate (1:200).
Western blot analysis of rat brain membrane (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell line lysate:1-2. Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (1:200).
3-4. Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
- Human live intact MEG-01 cells (5-10 µg antibody/1x106 cells).
- The blocking peptide in not suitable for this application.
- Rat basophilic leukemia (RBL) cells (1:100).
The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3.
The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.
The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.
Expression of P2Y12 Receptor in mouse BV-2 microglia cells.Cell surface detection of P2Y12 Receptor in live intact mouse BV-2 microglia cells using Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020). P2Y12 receptor immunoreactivity (green) is detected at the cell surface of cells.Adapted from Jiang, P. et al. (2017) PLoS ONE 12, e0183114. with permission of PLos.
