Overview
Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12 (Accession Q9H244). Intracellular, 2nd cytoplasmic loop.
Accession (Uniprot) Number Q9H244
Gene ID 64805
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 16/18 amino acid residues identical.
RRID AB_2040074.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, ip, wb
Western blot
- Rat brain membranes, human platelets (1:200).
Western blot analysis of rat brain membranes (1 and 2) and human platelets (3 and 4):1,3. Anti-P2Y12 Receptor Antibody (#APR-012), (1:200).
2,4. Anti-P2Y12 Receptor Antibody, preincubated with P2Y12 Receptor Blocking Peptide (#BLP-PR012).
Western blot analysis of human platelets:1. Anti-P2Y12 Receptor Antibody (#APR-012), (1:200)
2. Anti-P2Y12 Receptor Antibody, preincubated with P2Y12 Receptor Blocking Peptide (#BLP-PR012).
Immunoprecipitation
- Mouse bone marrow lysate (Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.).
Immunohistochemistry
- Mouse brain sections.
Immunocytochemistry
- Rat astrocytes (1:50) (Carrasquero, L.M. et al. (2005) Purinergic Signal. 1, 153.).
Indirect flow cytometry
- Rat osteoclasts (8 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The blocking peptide is not suitable for this application.
Scientific background
The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3
The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.
The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4
Lyophilized Powder
Image & Title: 
Expression of P2Y12 receptor in human eosinophils.A. Indirect flow cytometry of human eosinophils using Anti-P2Y12 Receptor Antibody (#APR-012). B. Immunocytochemical staining of human eosinophils using Anti-P2Y12 Receptor Antibody. P2Y12 immunostaining is shown in green. In both applications, preincubating the antibody with the blocking peptide abolishes the detection of the receptor.Adapted from Muniz, V.S. et al. (2015) PLoS ONE 10, e0139805. with permission of PLoS.
Expression of P2Y12 receptor in human eosinophils.A. Indirect flow cytometry of human eosinophils using Anti-P2Y12 Receptor Antibody (#APR-012). B. Immunocytochemical staining of human eosinophils using Anti-P2Y12 Receptor Antibody. P2Y12 immunostaining is shown in green. In both applications, preincubating the antibody with the blocking peptide abolishes the detection of the receptor.Adapted from Muniz, V.S. et al. (2015) PLoS ONE 10, e0139805. with permission of PLoS.
For research purposes only, not for human use
Last Update: 29/10/2024
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